Isolation of Shewanella putrefaciens GRD 03 from Fish and Explication of Biofilm Adherence Potency on Different Substrates

Foodborne pathogens are the main threat and cause of food poisoning. The majority infections have been related to biofilm formation foodborne in industry. Shewanella putrefaciens (KX355803, GRD 03), a Gram-negative pathogen isolated from mackerel fish, was identified recognized as spoilage bacterium strong producer. adhesion or attachment ability determined on steel, plastic, glass, PVC wood. NB (Nutrient broth), LB (Luria-Bertani TSB (Tryptic soy broth) BHI (Brain heart infusion were enriched with glucose shows optimum for bacterial adhesion. In microtiter plate method (MTP), observed at 48 72 hours incubation significant differences obtained p < 0.05. As period increases, OD value (Optical density) samples also increase. Biofilm is major cross-contamination, resistance certain disinfectants, which leads environmental stress tolerance. This study suggested production isolate fish by using media different substrates, comparing growth provide detailed idea about biofilm-forming time intervals.